In 1995, Sarah Connor, John Connor, Miles Dyson, and a T-800 destroyed the building with a massive amount of explosives in a preemptive strike against Skynet in order to destroy Dyson's work of Artificial intelligence and Neural Net CPU development. Dyson had to commit suicide by staying behind to trigger the detonator, which destroyed the lab so that the technology could not be used to create Skynet.
- While T-1000 is at the Dyson residence, it overhears on the police radio the dispatch ordering all available units to respond to the Cyberdyne Building and gives the address as "2144 Kramer St". In a later scene, as the police units are responding to the Cyberdyne Building, one of the officers asks the dispatch to repeat the address, she gives the address as "2111 Kramer".
- In several alternate timelines, such as Rise of the Machines timeline, Kyla's World, T2 Trilogy timeline, Jade's World and The Sarah Connor Chronicles timeline, the incident only delays the development of Skynet.
- In the ending of Ultimate version of Terminator 2: Judgment Day as well as Dark Fate timeline, the destruction of this building has caused the erasure of Skynet.
- Television series